The etiology of MC is unclear, although previous findings implicate luminal factors and thus the gut microbiome. Microscopic colitis (MC), an inflammatory disease of the colon, is characterized by chronic non-bloody diarrhea with characteristic inflammation and for some, collagen deposits in mucosal biopsies. concisus in the gastrointestinal tract is more extensive than previously assumed. concisus isolates from gut mucosal biopsies in both IBD patients and healthy controls than in preceding studies, indicating that colonization of C. concisus than healthy controls, by both cultivation and PCR detection. Patients with IBD had a higher prevalence of C. The rate of cultivation and PCR detection was higher for IBD patients compared to healthy controls (p = 0.021, p = 0.008, respectively). concisus, while 121/245 (49 %) of biopsies were PCR positive.

From 52 IBD patients, 52/245 (21 %) biopsies were culture positive for C. concisus isolates from mucosal biopsies than previously reported by other methods. We therefore investigated a novel two-step cultivation procedure combining anaerobic and microaerobic incubation from several gut mucosal sites to improve isolate yield, and compared this to PCR results, from IBD patients and healthy controls.Ĭultivation with the novel two-step procedure yielded a higher rate of C. Determining the rate of viable isolates present in the gut mucosa is of great importance when evaluating the role in different disease presentations. concisus culture-positive biopsies in previous studies has been very limited. It has been detected more often from intestinal biopsies in patients with IBD compared to healthy controls using PCR-based techniques, whereas the number of C. Campylobacter concisus is a commensal of the human oral flora that has been linked to prolonged diarrhea and inflammatory bowel disease (IBD).

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